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Objective@#To investigate the intake of liquid dairy products and identify its influencing factors among the fourth grade students in Nanjing City. @*Methods@#The fourth grade students were selected as the study subjects in Nanjing City using a multi-stage random cluster sampling method in September 2020. Their general information, frequency of liquid dairy product intake one week prior to survey and mean intake amount per time were collected. According to the 2016 Dietary Guidelines for Chinese Residents, weekly intake of liquid dairy products of 2 100 g and greater was defined eligible. Factors affecting the frequency and amount of liquid dairy product intake were identified using multivariable logistic regression analysis.@*Results@#A total of 2 268 questionnaires were allocated and 2 216 valid questionnaires were recovered, with an effective recovery rate of 97.71%. The respondents included 1 199 boys ( 54.11% ) and 1 017 girls ( 45.89% ). The frequency of liquid dairy product intake was (6.41±4.86) times per week, and the median intake amount was 1 250.00 g per week (interquartile range, 1 750.00 g per week). There were 607 students ( 27.39% ) consuming liquid dairy products of 2 100 g and greater a week, and 1 016 students ( 45.85% ) consumed liquid dairy products for 7 times and more a week. Multivariable logistic regression analysis showed that living in urban areas ( OR=1.204, 95%CI: 1.005-1.443 ), knowing nutrition labels ( OR=1.221, 95%CI: 1.021-1.460 ), periodical measurement of body weight (OR=1.486, 95%CI: 1.098-2.011) and restricted intake of sugar-containing drinks ( OR=1.264, 95%CI: 1.005-1.590 ) facilitated the intake of liquid dairy products for 7 times and more a week, and students with periodical measurement of body weight were more likely to consume liquid dairy products for 2 100 g and greater a week ( OR=1.821, 95%CI: 1.240-2.676 ).@*Conclusions@#Inadequate intake of liquid dairy products is found among the fourth grade students in Nanjing City. Residence, awareness of nutrition labels, periodical measurement of body weight, and parental restriction of sugar-containing drink intake affect the intake of liquid dairy products.
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Objective To summarize and analyze the clinical and epidemiological characteristics of 585 cases of cutaneous sporotrichosis collected in Jilin province in the last 3 years. Methods A retrospective study was conducted on sporotrichosis cases diagnosed at the Department of Dermatology in the hospital from 2007 to 2009. Results Totally, 585 cases were included in this study. Among these patients, the male/female ratio was 1:1.35 with an average age of 40.5 years and average duration of symptoms of 6.78 months.The age at onset was mainly between 51 and 60 years (22.05%). Sporotrichosis seemed more frequently to occur in winter and spring. The majority (94.19%) of the patients were rural habitants, and 149 (25.47%) patients recalled history of trauma. Fixed form was the most common clinical presentation (56.58%), followed by lymphocutaneous form (39.66%), cutaneous disseminated form ( 1.88% ) and undefined form ( 1.88% ). The predilection sites of sporotrichosis were extremities (50.94%) and face (43.76%). The treatment of sporotrichosis included a 10% solution of potassium iodide, itraconazole and terbinafine alone or in combination. Two hundred and fifty patients were lost to follow up. Of the remaining 335 patients, 289 were cured with an average treatment duration of 2.09 months, 46 were still under follow-up or treatment. Conclusions The case load of sporotrichosis in Jilin province has remained high in recent years. The clinical and epidemic features of sporotrichosis cases in this report are similar to those in previous reports, but the proportion of middle-aged patients and atypical cases increases. Potassium iodide solution, itraconazole and terbinafine are effective and safe for the treatment of sporotrichosis.
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Objective To study the histopathological changes and biological behavior of bowenoid papulosis and its relationship with apopotosis.Methods 20 bowenoid papuloses and 5 normal skin tissues were examined by DNA nick end labeling(TUNEL) technique and the pathological changes of bowenoid papulosis were observed by Hematoxylin-eosin-stained.Results Bowenoid papulosis exhibited disordered maturation and scattered,large,highly atypical cells mitoses throughout all layers of epithelium;and there were few apoptotic cells in 5 normal skin tissuese and many apoptotic cells in bowenoid papuloses.The apoptotic index(27.1?15.1) in bowenoid papulosis patients was significantly higher than that in control group(6.8?3.5)(P
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Cardio-protective action of synthetic peptide 6A, fibrinogen degradation segment, was observed on the myocardial injury model produced by subcutaneous injection of iso-proterenol (30 mg?kg-1-d-1) into rat. Treatment with peptide 6A (50 ?mol?kg-1? d-1,iv) significantly ameliorated isoproterenol-induced myocardial lesion, inhibited release of myocardial creatine phosphokinase, ?-hydroxybutyratedehydrogenase, lactate dehydrogenase and gluta-mate-oxaloacetate transaminase, lowered plasma fibrinogen content,and markedly prevented myocardial calcium accumulation. The results suggest that peptide 6A could have potential significance for clinical therapy of ischemia heart diseases.
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In the present experiment the high dose of LRH-A was injected into pregnant rats on Day 9 of pregnancy. Morphology and histochemistry of corpus luteum were studied. It was found that 24 hours after injection LRH-A could effectively cause the corpus luteum to degenarate. A great many large lipid droplets occured in the luteal cells, and the activity of 3?-HSD and of SDH of these cells decreased. At the same time the activity of AcP increased in a few of luteal cells. 48 hours after injection the effects outlined above became more distinct, and the activity of G-6-PD also began to decrease. The activity of 20 ?-HSD in both control and experimental groups was negative 24 hours after injection, while the activity of the AIP increased distinctly in endothelium of the capillary network of corpus luteum. These changes indicated that the high dose of LRH-A had damaged corpus luteum and reduced its synthetic and secretory function of progesterone which, we believe, plays a main role in terminating pregnancy.
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A murine MTEC_1 thymus epithelial cell line established by us has been characterized. The cells were polyhydral and closely packed each other as epithelial like cells. Using anti-keratin antibody, the keratin were shown in cytoplasm of all cells. Under electronmicroscope, bunches of tonofilaments were clearly shown in the cytoplasm, and desmosomes were seen between neighbouring cells. Using anti-mouse epithelial cell monoclonal antibodies for immunohistochemical study, nearly all of the MTEC_1 cells were MTS33 positive. It suggests that MTEC, cells were derived from the epithelial cells located in medulla. The majority of the MTEC_1 cells have normal mouse diploid chomosome number of 40. These results provide evidence that MTEC, cell line is normal murine thymus epithelial cells.
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The specific anti-rat LH, anti-rat FSH and anti-hCG sera were used in the peroxidase antiperoxidase (PAP) immunohistochemical method to observe the change of pituitary gonadotropic cells in pregnant rats after being injected by a high dosage of LHRH-A. It was observed that 24 hrs after injection the immunohistochemical reaction of gonadotropic cells weakened strikingly; 48 hrs after injection the immunohistochemical reaction began to recover; and 168 hrs after injection, the recovery was still in progress. The number of LH cells of different immunoreactive intensity and their percentage in total LH cells of the control and experimental groups were calculated and tested by statistical method. It was found that the results were the same as the above. Our observations support the view that the high dosage of LHRH-A can stimulate gonadotropic cells in rat to release a great amount of LH rapidly. It inhibits the synthesis of progesterone in the ovary of pregnant rat and results in termination of pregnancy. Moreover it is believed that the effect of high dosage of LHRH-A on the secretory function of gonadotropic cells can recover themseleves.
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12 cases of human placentas were obtained after administration of Chinese medicinal herb (Chuan-Xin-Lian) -induced abortion during mid-pregnancy. Six placentas from curettage induced abortion during mid-pregnancy were used as controls. All the specimens were studied histochemically and immunohistochemically by using antiserum to HCG with PAP method. In most of the specimens from women treated during mid-pregnancy, we observed a decreased immunoreaction of antiserum to HCG and a decrease of RNA content in the syncytiotrophoblasts. Increased activity of acid phosphatase in dccidual cells was observed during mid-pregnancy. These findings indicate that during mid-pregnancy, Chinese medicinal herb (Chuan-Xin-Lian) can reduce the synthesis of HCG in syncytiotrophoblasts and can initiate uterine contractions, so that abortion results. The possibility that the changes of lysosomal enzymes are involved in the initiation of uterine contractions is disscused.
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24 cases of human placentas were obtained from rivanol-induced abortion, and 6 cases from cesarean section, curettage, and water saccule-induced abortion were chosen for comparative study. All the specimens were proccessed under the same histological and histochemical procedures.In all the specimens from rivanol-induced abortion we observed a reduction in the activity of the ?~5-3?-hydroxysteroid dehydrogenase and of the number of fine lipid droplets in the syncytiotrophoblasts, and in these cells we also observed an increased activity in acid phosphatase in most of the specimens and a decrease of RNA content in a few specimens. A large number of decidual cells and a few trophoblasts showed necrotic changes. The chorionic plate also showed necrosis and distinct inflammatory reactions, and on the fetal surface of the placenta the blood circulation showed disturbance. These changes indicated that rivanol had demaged the placenta and reduced its synthetic function of progesterone which we believed played a rote in terminating pregnancy and caused abortion eventually. It was suggested that the intraamniotic injection of rivanol is preferable to extraamniotic injection.